The Nature of the Ratio of B1 to B2 Lymphocytes in Patients with Common Variable Immunodeficiency - European Medical Journal

The Nature of the Ratio of B1 to B2 Lymphocytes in Patients with Common Variable Immunodeficiency

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Allergy & Immunology
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EMJ Allergy & Immunology 6.1 Feature Image
*Ludmila Sizyakina,1 Maria Kharitonova,1 Irina Andreeva1

The authors have declared no conflicts of interest.


The authors would like to thank the enrolled patients for participating in this study.

EMJ Allergy and Immunology. ;6[1]:41-42.
B1 and B2 lymphocytes, common variable immunodeficiency (CVID).

Each article is made available under the terms of the Creative Commons Attribution-Non Commercial 4.0 License.


Antibody production inhibition is a leading sign of common variable immunodeficiency (CVID), but the causes of this defect are not fully disclosed.1,2 It is known that the basis of the violation of antibody synthesis results in a change in the functions of B lymphocytes. At the same time, the data on the quantitative and functional features of B lymphocyte subpopulations in CVID are very contradictory, which determines the interest in this area of research.3,4


To identify a characteristic of the B1 and B2 subpopulations of lymphocytes in patients with CVID.


Ten patients aged 28–62 years (male: 4; female: 6) with a diagnosis of CVID were followed up. All the patients received intravenous Ig therapy (0.4 g/kg of body weight, monthly). The data of the examination before the next infusion in the conditions of clinical remission were analysed. The control group consisted of 10 blood donors. The phenotypic parameters of B lymphocytes were evaluated by flow cytofluorimetry using appropriate sets of monoclonal antibodies.


It is known that B lymphocytes of the peripheral blood are mainly represented by the B2 subpopulation, and the increase in the content of B1 lymphocytes that are ontogenetically, phenotypically, and functionally different from them is a reflection of intra-immune dysregulation.5 The results of the subpopulation characteristics of B cells of the control group showed that the proportion of B2 lymphocytes was 97.5% (95–99) with a ratio of B1:B2 of 1:40. In the cohort of patients with CVID, the B2 phenotype averaged 85.7% (77–92) of all circulating B lymphocytes. In this case, the ratio of B1:B2 in 100% of cases was lower than the data of the comparison group, but the spread of values varied very significantly, from 1:3 to 1:31. Comparison of the results of phenotyping of B cells with the data of the serum IgG revealed that the values of the ratio B1:B2 closest to the control (1:26; 1:22–1:31]), observed in patients with IgG pre-transfusion level of 8.1 g/L (7.9–8.3), whereas in the group of patients with CVID, where the ratio of B1:B2 in relation to control reduced significantly (1:7; 1:3–1:9), pre-transfusion values for IgG ranged from 6.8 (6.5–7.1) g/L.


Thus, a decrease in the proportion of B2 lymphocytes in the total pool of circulating B cells was common for all the presented observations of patients with CVID, and the variability in the degree of decrease correlated with the pre-transfusion level of IgG content.

Abbott JK, Gelfand EW. Common variable immunodeficiency: diagnosis, management, and treatment. Immunol Allergy Clin North Am. 2015;35(4):637-58. Tangye SG et al. Human inborn errors of immunity: 2019 update on the classification from the international union of immunological societies expert committee. J Clin Immunol. 2020;40(1):24-64. Suchanek O. et al. Immunophenotyping of putative human B 1 B cells in healthy controls and CVID patients. Clin Exp Immunol. 2012;170(3):333-41. Kraljevic K. et al. Circulating phenotypic B-1 cells are decreased in common variable immunodeficiency and correlate with immunoglobulin M levels. Clin Exp Immunol. 2012;171(3):278-82. Quách TD et al. Distinctions among circulating antibody-secreting cell populations, including B-1 Cells, in human adult peripheral blood. J Immunol. 2016;196(3):1060-9.

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